The trial will be open to 315 people over the next 15 months.
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The trial will commence later this year and grant 2,000 people access to the HIV-prevention pill.
The state government has been ‘strongly resistant’ to the trial, saying the pool of participants would be too small to run one.
Not only will PrEP be available in Norway, it will be free for those at risk of acquiring HIV
While no HIV prevention tool is perfect, experts have still stressed the benefits of PrEP despite the two cases this year.
More than 1,300 same-sex attracted men have now signed up to the Victorian study.
The Pharmaceutical Benefits Advisory Committee declared Truvada would not be cost effective for the Australian population.
The party has pledged to support people at high-risk of HIV.
Truvada, which is used to prevent HIV, has been approved in Australia by the Therapeutic Goods Administration (TGA).